Forensic

  Presented By:
           
  Forensic
  Promega:
           
  Promega

   

Speakers:


Speaker


Moderator:
 
Rebecca Waters

Editor-in-Chief
Forensic Magazine

 


Speaker


Speaker:

Professor Lutz Roewer

PhD Institute of Legal Medicine and Forensic Sciences, Department of Forensic Genetics

 

An Overview of the Global PPY23-YHRD Database Project and the Impact on the Forensics Workflow


Date: Thursday, October 24th
Time: 9:00 am CST
Duration: 60 Minutes


In this webinar, we will discuss the results from a large collaborative project initiated in September 2012 to collect, analyze, database and publish population data of 23 Y-STR locus haplotypes (PowerPlex® Y23 System which was released in August 2012). As many as 18,863 haplotypes from 127 populations were gathered within the 8 months of the collection phase of the project. Seventy-one institutions from 33 countries submitted data and participated in an ad hoc quality assessment organized by the YHRD which included blind testing of DNA samples. Allele and haplotype frequencies as well as diversities were calculated per country, ethnic group and continent. Observations on non-uniform alleles were collected and sequence information was gathered. Genetic relationships between different population groups were quantified by means of RST, thereby taking the evolutionary distance between individual Y-STR haplotypes into account. The discrimination indices were compared for AmpFLSTR® Yfiler® PCR Amplification Kit and PowerPlex® Y23 System profiles. The YHRD functionality was updated to enable searches in the PowerPlex® Y23 format. The creation of a large global reference database for the PowerPlex® Y23 haplotype format assists forensic labs with the implementation of high-resolution Y chromosome diagnostics in their workflow.

 
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